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Infectious Disease: Coronavirus

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Insulated Isothermal Reverse Transcriptase PCR (iiRT ‐PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus
In this study, we describe validation of a new probe‐based insulated isothermal reverse transcriptase PCR (iiRT‐PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user‐friendly device (POCKIT™ Nucleic Acid Analyzer) that does not need data interpretation by the user. The assay accurately detected CSFV RNA from a diverse panel of 33 CSFV strains representing all three genotypes plus an additional in vitro‐transcribed RNA from cloned sequences representing a vaccine strain. No cross‐reactivity was observed with a panel of 18 viruses associated with livestock including eight other ...
Source: Transboundary and Emerging Diseases - January 26, 2015 Category: Veterinary Research Authors: O. Lung, J. Pasick, M. Fisher, C. Buchanan, A. Erickson, A. Ambagala Tags: Original Article Source Type: research

Stage of Gestation at Porcine Epidemic Diarrhea Virus Infection of Pregnant Swine Impacts Maternal Immunity and Lactogenic Immune Protection of Neonatal Suckling Piglets
In this study, we infected pregnant first parity gilts in their first, second and third trimesters of gestation with PEDV to determine the impact of stage of gestation on generation of maternal B-cell immunity, the gut-MG-sIgA axis and lactogenic immune protection in PEDV challenged piglets. Our goal was to identify innate and adaptive immune factors during pregnancy that influence lymphocyte trafficking, in addition to immune correlates of lactogenic immune protection in neonatal suckling piglets. Understanding the impact of stage of gestation at PEDV infection or exposure on maternal immunity will allow more precise mate...
Source: Frontiers in Immunology - April 23, 2019 Category: Allergy & Immunology Source Type: research

TWiV 582: This little virus went to market
TWiV provides updates on the new coronavirus causing respiratory disease in China, the current influenza season, and the epidemic of African swine fever, including determination of the three-dimensional structure of the virus particle. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, Kathy Spindler, and Brianne Barker Download TWiV 582 (71 MB .mp3, 118 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode ASV 2020 NJ ready to remove religious vaccine exemptions (Patch) New virus causing pneumonia in China (NY Times) New China virus is coronavirus (NY ...
Source: This Week in Virology - MP3 Edition - January 12, 2020 Category: Virology Authors: Vincent Racaniello Source Type: podcasts

Swine enteric coronavirus disease: A review of 4  years with porcine epidemic diarrhoea virus and porcine deltacoronavirus in the United States and Canada
Summary Swine enteric coronaviruses, including porcine epidemic diarrhoea virus (PEDV) and porcine deltacoronavirus (PDCoV), have emerged and spread throughout the North American swine industry over the last four years. These diseases cause significant losses within the pork industry and within the first year after PEDV introduction, approximately 10% of the US herd died due to the disease. Similar to other enteric coronaviruses, such as transmissible gastroenteritis virus (TGEV), these emerging swine enteric coronavirus diseases (SECD) are age‐dependent, with high morbidity and mortality in neonatal pigs. Since the intr...
Source: Transboundary and Emerging Diseases - February 2, 2018 Category: Veterinary Research Authors: M. C. Niederwerder, R. A. Hesse Tags: REVIEW ARTICLE Source Type: research

Swine enteric alphacoronavirus (swine acute diarrhea syndrome coronavirus): an update three years after its discovery.
Abstract Discovered in 2017, swine enteric alphacoronavirus (SeACoV), also known as swine acute diarrhea syndrome coronavirus (SADS-CoV) or porcine enteric alphacoronavirus (PEAV), is the fifth porcine CoV identified in diarrheal piglets. The presumed name "SADS-CoV" may not be appropriate since current studies have not provided strong evidence for high pathogenicity of the virus. SeACoV was the most recently recognized CoV of potential bat origin prior to the novel human severe acute respiratory syndrome CoV 2 (SARS-CoV-2), associated with the pandemic CoV disease 2019 (COVID-19). Although SeACoV is recognized as...
Source: Virus Research - May 15, 2020 Category: Virology Authors: Yang YL, Yu JQ, Huang YW Tags: Virus Res Source Type: research

Identification and epitope mapping of swine acute diarrhea syndrome coronavirus accessory protein NS7a via monoclonal antibodies
Virus Res. 2022 Mar 10:198742. doi: 10.1016/j.virusres.2022.198742. Online ahead of print.ABSTRACTSwine acute diarrhea syndrome coronavirus (SADS-CoV) is an emerging swine enteric coronavirus that causes vomiting, severe diarrhea, dehydration and death in suckling piglets. NS7a is putative accessory protein that is predicted to be encoded by SADS-CoV, but still to be confirmed experimentally. In the present study, recombinant NS7a protein was expressed in a prokaryotic expression system and used as an antigen to prepare monoclonal antibodies (mAbs) specific to NS7a protein. We obtained two anti-NS7a mAbs, termed AH5 and EH...
Source: Virus Research - March 14, 2022 Category: Virology Authors: Yibin Qin Tingshuai Feng Hongyan Shi Jiyu Zhang Liaoyuan Zhang Shufeng Feng Jianfei Chen Ying He Xin Zhang Zhongwei Chen Jianbo Liu Dakai Liu Da Shi Li Feng Source Type: research

Swine coronaviruses (SCoVs) and their emerging threats to swine population, inter-species transmission, exploring the susceptibility of pigs for SARS-CoV-2 and zoonotic concerns
Vet Q. 2022 May 18:1-39. doi: 10.1080/01652176.2022.2079756. Online ahead of print.ABSTRACTSwine coronaviruses (SCoVs) are one of the most devastating pathogens affecting the livelihoods of farmers and swine industry across the world. These include transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine respiratory coronavirus (PRCV), porcine hemagglutinating encephalomyelitis virus (PHEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), and porcine delta coronavirus (PDCoV). Coronaviruses infect a wide variety of animal species and humans because these are having single stranded-R...
Source: Veterinary Quarterly - May 18, 2022 Category: Veterinary Research Authors: Jigarji C Thakor Murali Dinesh Rajendran Manikandan Suresh Bindu Monalisa Sahoo Diptimayee Sahoo Manish Dhawan Megha Katare Pandey Ruchi Tiwari Talha Bin Emran Kuldeep Dhama Wanpen Chaicumpa Source Type: research